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girk2 rabbit alomone apc  (Alomone Labs)


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    Alomone Labs girk2 rabbit alomone apc
    Girk2 Rabbit Alomone Apc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+girk2/Anti-GIRK2+(Kir3%2E2)+Antibody/us12428623-703-143-145
    Average 94 stars, based on 84 article reviews
    girk2 rabbit alomone apc - by Bioz Stars, 2026-09
    94/100 stars

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    Staining:

    Article Title: Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development
    Article Snippet: .. KD cultures were fixed and stained with rabbit anti-TH (1:1000, #ab152; Millipore), mouse anti-TH (1:300, #MAB318; Sigma-Aldrich), mouse anti-Calb1 (1:500, #C9848; Sigma), Rabbit anti-Girk2 (1:500, #APC-006, Alomone Labs), Rabbit anti-Nurr1 (1:10–1:20, #PA5-13416; Invitrogen), Rabbit anti-En1 (1:100, #BS-11744R; Bioss), or chicken anti-GFP (1:1000, #GFP-1020; Aves Labs). .. For fluorescent labelling, secondary antibodies Alexa Fluor 488 Donkey anti-chicken (1:500–1:750, #703-545-155, Jackson Immuno Research), Alexa Fluor 568 Donkey anti-mouse (1:500–1:750, #A10037; Invitrogen), Alexa Fluor 568 Donkey anti-rabbit (1:500–1:750, #ab175470; Abcam), Alexa Fluor 647 Donkey anti-mouse (1:500–1:750, #A-31571; Invitrogen), Alexa Fluor 647 Donkey anti-Rabbit (1:500–1:750, #A32795; Thermo Scientific), Alexa Fluor 488 Goat anti-rabbit (1:750, #Ab150077; Abcam), Alexa Fluor 568 Goat anti-mouse (1:750, #A11004; Thermo Scientific), Alexa Fluor 647 Goat anti-chicken (1:750, #A21449; Invitrogen) and Alexa Fluor 488 Goat anti-chicken (1:750, #Ab150169; Abcam) were applied.

    Article Title: Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development.
    Article Snippet: .. KD cultures were fixed and stained with rabbit anti-TH (1:1000, #ab152; Millipore), mouse anti-TH (1:300, #MAB318; Sigma-Aldrich), mouse anti-Calb1 (1:500, #C9848; Sigma), Rabbit anti-Girk2 (1:500, #APC-006, Alomone Labs), Rabbit anti-Nurr1 (1:10–1:20, #PA5-13416; Invitrogen), Rabbit anti-En1 (1:100, #BS-11744R; Bioss), or chicken antiGFP (1:1000, #GFP-1020; Aves Labs). .. For fluorescent labelling, secondary antibodies Alexa Fluor 488 Donkey anti-chicken (1:500–1:750, #703-545-155, Jackson Immuno Research), Alexa Fluor 568 Donkey anti-mouse (1:500–1:750, #A10037; Invitrogen), Alexa Fluor 568 Donkey anti-rabbit (1:500–1:750, #ab175470; Abcam), Alexa Fluor 647 Donkey anti-mouse (1:500–1:750, #A-31571; Invitrogen), Alexa Fluor 647 Donkey anti-Rabbit (1:500–1:750, #A32795; Thermo Scientific), Alexa Fluor 488 Goat anti-rabbit (1:750, #Ab150077; Abcam), Alexa Fluor 568 Goat anti-mouse (1:750, #A11004; Thermo Scientific), Alexa Fluor 647 Goat anti-chicken (1:750, #A21449; Invitrogen) and Alexa Fluor 488 Goat anti-chicken (1:750, #Ab150169; Abcam) were applied.

    Incubation:

    Article Title: Postsynaptic adaptations in direct pathway muscarinic M4-receptor signaling follow the temporal and regional pattern of dopaminergic degeneration.
    Article Snippet: .. Sliceswere then incubated with rabbit anti-GIRK2 (1:500, Alomone labs APC-006, RRID: AB_2040115) and goat anti-ChAT (1:400, Milipore AB144P, RRID: AB_2079751) primary antibodies overnight at 4°C on shaker. .. After washes with 0.5%PBS-T, slices were incubatedwith donkey anti-rabbit Alexa Fluor 488 (1:500, Life Technologies A21206, RRID: AB_2535792) and donkey anti-goat Alexa Fluor 647 (1:500, Abcam ab150131, RRID:AB_2732857) secondary antibodies for 1 h at RT on shaker and washed afterward with PBS.

    Article Title: Postsynaptic adaptations in direct pathway muscarinic M4-receptor signaling follow the temporal and regional pattern of dopaminergic degeneration
    Article Snippet: .. Slices were then incubated with rabbit anti-GIRK2 (1:500, Alomone labs APC-006, RRID: AB_2040115) and goat anti-ChAT (1:400, Milipore AB144P, RRID: AB_2079751) primary antibodies overnight at 4°C on shaker. .. After washes with 0.5% PBS-T, slices were incubated with donkey anti-rabbit Alexa Fluor 488 (1:500, Life Technologies A21206, RRID: AB_2535792) and donkey anti-goat Alexa Fluor 647 (1:500, Abcam ab150131, RRID:AB_2732857) secondary antibodies for 1 h at RT on shaker and washed afterward with PBS.

    Article Title: Midbrain dopamine (DA) neurons for engraftment
    Article Snippet: .. Immunohistological and stereological procedures: Free floating coronal sections were blocked in 0.1% Triton X-100, 5% donkey serum in PBS for 30 min at room temperature and incubated 48 hrs at 4° C. with different antibodies: rabbit anti-GFP (1:300), chicken anti-GFP (1:200, Chemicon), mouse anti-TH (1:200), mouse IgM anti-PSA (1:1000), mouse anti-NeuN (1:400), goat anti-FoxA2 (1:300), rabbit anti-Girk2 (1:300, Alomone Labs), mouse anti-synapsin (1:200, BD Transduction Laboratories). ..

    Article Title: Postsynaptic adaptations in direct pathway muscarinic M4-receptor signaling follow the temporal and regional pattern of dopaminergic degeneration
    Article Snippet: .. Slices were then incubated with rabbit anti-GIRK2 (1:500, Alomone labs APC-006, RRID: AB_2040115) and goat anti-ChAT (1:400, Milipore AB144P, RRID: AB_2079751) primary antibodies overnight at 4°C on shaker. .. After washes with 0.5% PBS-T, slices were incubated with donkey anti-rabbit Alexa Fluor 488 (1:500, Life Technologies A21206, RRID: AB_2535792) and donkey anti-goat Alexa Fluor 647 (1:500, Abcam ab150131, RRID:AB_2732857) secondary antibodies for 1 h at RT on shaker and washed afterward with PBS.



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    Fig. 2. Increased efficiency of <t>GABAB/Girk2</t> signaling in DG granule cells of neonatal Ts65Dn mice compared to 2N controls. A. Representative whole-cell voltage-clamp recordings from 2N and Ts65Dn granule cells showing neuronal input resistance before (black traces) and during (gray traces) bac lofen application. Voltage steps (−10 mV for 500 ms) were applied from a holding potential of −70 mV, and input resistance was calculated using Ohm’s law. B. Changes in normalized input resistance in 2N and Ts65Dn neurons during baclofen application. C. Quantification of the data showing that the reduction in input resistance induced by baclofen was significantly greater in neurons from Ts65Dn pups (n = 4 neurons,4 mice) compared to 2N pups (n = 7 neurons, 5 mice). Data are presented as mean ± SD. *P < 0.05 (Student’s t-test).
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    Fig. 2. Increased efficiency of <t>GABAB/Girk2</t> signaling in DG granule cells of neonatal Ts65Dn mice compared to 2N controls. A. Representative whole-cell voltage-clamp recordings from 2N and Ts65Dn granule cells showing neuronal input resistance before (black traces) and during (gray traces) bac lofen application. Voltage steps (−10 mV for 500 ms) were applied from a holding potential of −70 mV, and input resistance was calculated using Ohm’s law. B. Changes in normalized input resistance in 2N and Ts65Dn neurons during baclofen application. C. Quantification of the data showing that the reduction in input resistance induced by baclofen was significantly greater in neurons from Ts65Dn pups (n = 4 neurons,4 mice) compared to 2N pups (n = 7 neurons, 5 mice). Data are presented as mean ± SD. *P < 0.05 (Student’s t-test).
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    Image Search Results


    Fig. 2. Increased efficiency of GABAB/Girk2 signaling in DG granule cells of neonatal Ts65Dn mice compared to 2N controls. A. Representative whole-cell voltage-clamp recordings from 2N and Ts65Dn granule cells showing neuronal input resistance before (black traces) and during (gray traces) bac lofen application. Voltage steps (−10 mV for 500 ms) were applied from a holding potential of −70 mV, and input resistance was calculated using Ohm’s law. B. Changes in normalized input resistance in 2N and Ts65Dn neurons during baclofen application. C. Quantification of the data showing that the reduction in input resistance induced by baclofen was significantly greater in neurons from Ts65Dn pups (n = 4 neurons,4 mice) compared to 2N pups (n = 7 neurons, 5 mice). Data are presented as mean ± SD. *P < 0.05 (Student’s t-test).

    Journal: Experimental neurology

    Article Title: Early postnatal GABAB antagonist treatment normalizes inhibitory/excitatory balance in neonatal Ts65Dn mice, a genetic model of down syndrome.

    doi: 10.1016/j.expneurol.2025.115171

    Figure Lengend Snippet: Fig. 2. Increased efficiency of GABAB/Girk2 signaling in DG granule cells of neonatal Ts65Dn mice compared to 2N controls. A. Representative whole-cell voltage-clamp recordings from 2N and Ts65Dn granule cells showing neuronal input resistance before (black traces) and during (gray traces) bac lofen application. Voltage steps (−10 mV for 500 ms) were applied from a holding potential of −70 mV, and input resistance was calculated using Ohm’s law. B. Changes in normalized input resistance in 2N and Ts65Dn neurons during baclofen application. C. Quantification of the data showing that the reduction in input resistance induced by baclofen was significantly greater in neurons from Ts65Dn pups (n = 4 neurons,4 mice) compared to 2N pups (n = 7 neurons, 5 mice). Data are presented as mean ± SD. *P < 0.05 (Student’s t-test).

    Article Snippet: Membranes were then incubated overnight at 4 ◦C with the primary rabbit polyclonal anti-Girk2 antibody (1:1′000, Alomone Labs, Cat # APC-006, Lot # APC006AN2025).

    Techniques: